Abstract
BACKGROUND: Kukkutand Tvak (egg’s shell) is an important drug of Ayurveda and Unani Medicine. In the literature of Rasashastra (branch of Ayurveda chemistry) it is classified under Sudha Varga (group of Calcium containing drugs) and it contains Calcium carbonate up to 90% along with other trace elements like phosphates, magnesium, calcium etc. It is a very cheap source of calcium for human nutrition and many previous research works have proved that Kukkutanda Tvak Bhasma (KTB) minimises the risk of Asthikshay (osteoporosis), Shwetpradar (Leucorrhoea) etc. In classical texts, several number of Bhavana Dravya (levigating material) are advised for the preparation of KTB which enhances its potency. AIM: An attempt has been made to prepare KTB with Bhavana of Asthisamharaka (Cissus quadrangularis Linn) during Marana (incineration). METHODOLOGY: In the present study, Asthisamharka Swaras (juice) as well as Kumari Swaras (Aloe vera juice) is used for the Bhavana Process. Pharmaceutical standardisation was carried out in three batches and parameter for quality assurance was also studied. Analytical study was done to establish the basic standard for KTB. RESULTS: After six alternate Bhavana of Asthisamharka and Kumari Swaras followed with four Putas applying at 850 degree C temperature in Electrical muffle furnace (EMF), After fourth Puta bright white colour, very fine, soft powder, without specific smell obtain Conclusion: Kukkutanda Tvak Bhasma is rich source of calcium was given Bhavana of Asthisamharka and Kumari Swaras to improve its potency. After four Puta it yielded 66.4% Bhasma and 33.6 % weight loss was found. CONCLUSION: As there is no pharmacopeia standard guideline, the analytical findings of present study can be considered for future research and also there is a need to evaluate the efficacy of this formulation as an Asthiposhak (Strength to Asthi Dhatu) in condition of calcium deficiency and osteoporosis.

Key words: Asthisamharak, Bhavana, Incinerated egg shell, Kukkutanda Tvak Bhasma