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Assessment of mutagenicity of Boswellia serrata extract (Boswegex®) using Bacterial Reverse Mutation test method

Bhardwaj Gururaj Vadiraj, Chepattu Mohammed Abdullah Anzar, Molath Varkey Joseph, Padmanabhan Chettayil Prasad.


Abstract
Background: Extracted from the Indian branching tree Boswellia serrata, boswellic acids derived from the plant's resin hold promise in addressing inflammation, arthritis, and hyperlipidemia. Objective: In this study, the mutagenicity of B. serrata (Boswegex®) is evaluated. Materials and Methods: The potential genotoxic and mutagenic effects of Boswellia serrata's aqueous ethanolic extract, known as Boswegex®, were explored using a bacterial reverse mutation assay against Salmonella strains TA98, TA100, TA102, TA1535, and TA1537. Different concentrations ranging from 500 to 6000 μg/plate were tested using both Method 1 (Standard plate incorporation assay) and Method 2 (pre-incubation assay), with DMSO (Dimethyl sulfoxide) as the solvent control. The positive control, 2-aminoanthracene, was used alongside a metabolic activator (S-9 fraction). Given the prolonged use of supplements, an assessment for mutagenicity is vital. In this context, the mutagenicity of B. serrata (Boswegex®) was evaluated through the in vitro Bacterial Reverse Mutation Test, employing both the Standard Plate Incorporation Assay and the pre-incubation assay. Results: Notably, none of the mutagenic assays revealed a mutagenicity increase over background levels. Conclusion: These findings collectively indicate that Boswellia serrata (Boswegex®) does not exhibit mutagenic properties.

Key words: Bacterial Reverse Mutation Test, Boswellia serrata (Boswegex®), pre-incubation assay, Standard plate incorporation assay


 
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How to Cite this Article
Pubmed Style

Vadiraj BG, Anzar CMA, Joseph MV, Prasad PC. Assessment of mutagenicity of Boswellia serrata extract (Boswegex®) using Bacterial Reverse Mutation test method. J. res. tradit. med. 2023; 9(July-December 2023): 34-43. doi:10.5455/jrtm.2023/165552


Web Style

Vadiraj BG, Anzar CMA, Joseph MV, Prasad PC. Assessment of mutagenicity of Boswellia serrata extract (Boswegex®) using Bacterial Reverse Mutation test method. https://www.tmjournal.org/?mno=165552 [Access: June 07, 2024]. doi:10.5455/jrtm.2023/165552


AMA (American Medical Association) Style

Vadiraj BG, Anzar CMA, Joseph MV, Prasad PC. Assessment of mutagenicity of Boswellia serrata extract (Boswegex®) using Bacterial Reverse Mutation test method. J. res. tradit. med. 2023; 9(July-December 2023): 34-43. doi:10.5455/jrtm.2023/165552



Vancouver/ICMJE Style

Vadiraj BG, Anzar CMA, Joseph MV, Prasad PC. Assessment of mutagenicity of Boswellia serrata extract (Boswegex®) using Bacterial Reverse Mutation test method. J. res. tradit. med. (2023), [cited June 07, 2024]; 9(July-December 2023): 34-43. doi:10.5455/jrtm.2023/165552



Harvard Style

Vadiraj, B. G., Anzar, . C. M. A., Joseph, . M. V. & Prasad, . P. C. (2023) Assessment of mutagenicity of Boswellia serrata extract (Boswegex®) using Bacterial Reverse Mutation test method. J. res. tradit. med, 9 (July-December 2023), 34-43. doi:10.5455/jrtm.2023/165552



Turabian Style

Vadiraj, Bhardwaj Gururaj, Chepattu Mohammed Abdullah Anzar, Molath Varkey Joseph, and Padmanabhan Chettayil Prasad. 2023. Assessment of mutagenicity of Boswellia serrata extract (Boswegex®) using Bacterial Reverse Mutation test method. JOURNAL OF RESEARCH IN TRADITIONAL MEDICINE, 9 (July-December 2023), 34-43. doi:10.5455/jrtm.2023/165552



Chicago Style

Vadiraj, Bhardwaj Gururaj, Chepattu Mohammed Abdullah Anzar, Molath Varkey Joseph, and Padmanabhan Chettayil Prasad. "Assessment of mutagenicity of Boswellia serrata extract (Boswegex®) using Bacterial Reverse Mutation test method." JOURNAL OF RESEARCH IN TRADITIONAL MEDICINE 9 (2023), 34-43. doi:10.5455/jrtm.2023/165552



MLA (The Modern Language Association) Style

Vadiraj, Bhardwaj Gururaj, Chepattu Mohammed Abdullah Anzar, Molath Varkey Joseph, and Padmanabhan Chettayil Prasad. "Assessment of mutagenicity of Boswellia serrata extract (Boswegex®) using Bacterial Reverse Mutation test method." JOURNAL OF RESEARCH IN TRADITIONAL MEDICINE 9.July-December 2023 (2023), 34-43. Print. doi:10.5455/jrtm.2023/165552



APA (American Psychological Association) Style

Vadiraj, B. G., Anzar, . C. M. A., Joseph, . M. V. & Prasad, . P. C. (2023) Assessment of mutagenicity of Boswellia serrata extract (Boswegex®) using Bacterial Reverse Mutation test method. JOURNAL OF RESEARCH IN TRADITIONAL MEDICINE, 9 (July-December 2023), 34-43. doi:10.5455/jrtm.2023/165552